Pharmacokinetic parameters determination

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Ionization constant (pKa) and partition coefficient (log P) evaluation.
Physico-chemical properties (ionization constant and partition coefficient) are crucial for biodistribution, metabolism and escretion of a drug.
These properties are experimentally determined through different methodologies:
Potentiometric titrations at 25 °C in pH range 2-12;
“Shake-flask” method with organic solvents (n-octanol, cyclohexane, CHCl3) and phosphate buffer pH 7.4 and subsequent spectophotometric UV quantitative analysis.
Available Facilities:
Sirius Analytical GLpKa
PerkinElmer UV-Vis-NIR Spectrophotometer